Tips for Chemiluminescence Detection: Western Blotting Edition
We will introduce some detailed points of caution regarding experimental methods such as swimming motion and antibody detection.
The "Western blotting method" is a technique used to specifically detect target proteins by transferring protein samples onto a membrane after electrophoresis and using specific antibodies. This document, "Tips for Chemiluminescence Detection: Western Blotting Edition," outlines the use of the chemiluminescence imaging device "AE-6971/2 Light Capture" for detection, as well as detailed precautions regarding experimental methods such as electrophoresis and antibody detection. [Support for resolving the following issues] ■ Regarding band detection status: - The target band is faint and cannot be confirmed. - High background or unevenness prevents confirmation of the target band. ■ Issues with capturing luminescence patterns: - Adjusting exposure with X-ray film is difficult. - X-ray film makes data reading and analysis challenging. For more details, please contact us or download the catalog.
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